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Objective-To examine whether in vitro treatment with trans-10, cis-12 conjugated linoleic acid (t10c12-CLA) restores the phagocytic capacity and oxidative burst activity (OBA) of canine polymorphonuclear neutrophilic leukocytes (PMNs) exposed to methylprednisolone sodium succinate (MPSS). Sample Population-Peripheral blood PMNs obtained from 12 healthy Beagles. Procedures-The experimental design involved administration of a high dose of MPSS, which is the recommended protocol for dogs with acute spinal cord injury. To evaluate PMN function, blood samples were collected from dogs before IV injections of doses of MPSS or saline (0.9% NaCl) solution (time 0) and 2, 12, and 24 hours after injections ceased. Polymorphonuclear neutrophilic leukocytes were isolated from blood samples and incubated with t10c12-CLA alone or t10c12-CLA in combination with N-acetylcysteine (an antioxidant agent). Phagocytic capacity and OBA were measured simultaneously by use of flow cytometry. Results-The phagocytic capacity and OBA of PMNs were suppressed by IV injection of MPSS and restored 12 hours after injection ceased. In vitro treatment with t10c12-CLA enhanced the phagocytic capacity and OBA of PMNs, regardless of whether dogs had been treated with MPSS. Effects of t10c12-CLA on OBA were detected only when phagocytosis was stimulated by microspheres. Use of N-acetylcysteine attenuated the stimulatory effects of t10c12-CLA. Conclusions and Clinical Relevance-Exposure to t10c12-CLA enhanced the phagocytic capacity and OBA of canine PMNs, and this effect may have involved t10c12-CLA-induced generation of reactive oxygen species.
Kang JH Yang MP
Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
Background To evaluate the efficacy of antegrade insertion of the ureteric stent using pull-through technique in tight ureteric stricture that a guide-wire traversed, but a 5-F catheter could not.
Byung Moon Kim2 Email:bmoon21@hanmail.net%0A Sung Il Park1 Email:astarte@empal.com
[1] Department of Diagnostic Radiology, Kwandong University Myongji Hospital, Koyang-si, Republic of Korea ;[2] Department of Diagnostic Radiology, Kangbuk Samsung Hospital, Sungkyunkwan University, Seoul, Republic of Korea
2008,33,000,1,2
BACKGROUND: The aims of this study are to evaluate and compare the accuracies of isotropic multi-detector row CT (MDCT) including multiplanar reconstruction (MPR) images for preoperative staging of endoscopically diagnosed early gastric cancer (eEGC) and advanced gastric cancer (eAGC). METHODS: One hundred and five patients with endoscopically proven gastric cancer underwent isotropic MDCT. Three independent radiologists evaluated T and N staging both on transverse images only and on three orthogonal MPR images. The staging of each tumor was surgico-pathologically confirmed. The receiver operating characteristic (ROC) analysis and the Wilcoxon signed ranked test were used for statistical analyses. RESULTS: In 30 eAGCs, the accuracies for T and N staging with MPR imaging were better than those with transverse imaging (each P < 0.05). In 34 eEGCs, however, only the accuracy of T2 or higher with MPR imaging was higher than that with transverse imaging (P < 0.05). CONCLUSIONS: Isotropic MDCT with MPR images including coronal or sagittal reconstructions can improve the accuracies of preoperative T and N staging of eAGC, while having little impact on the accuracy for eEGC.
Kim YN Choi D Kim SH Kim MJ Lee SJ Lee WJ Kim S Kim JJ
Department of Radiology and Center for Imaging Science, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Ilwon-dong, Kangnam-Ku, Seoul, 135-710, Republic of Korea.
BACKGROUND: The aims of this study are to evaluate and compare the accuracies of isotropic multi-detector row CT (MDCT) including multiplanar reconstruction (MPR) images for preoperative staging of endoscopically diagnosed early gastric cancer (eEGC) and advanced gastric cancer (eAGC). METHODS: One hundred and five patients with endoscopically proven gastric cancer underwent isotropic MDCT. Three independent radiologists evaluated T and N staging both on transverse images only and on three orthogonal MPR images. The staging of each tumor was surgico-pathologically confirmed. The receiver operating characteristic (ROC) analysis and the Wilcoxon signed ranked test were used for statistical analyses. RESULTS: In 30 eAGCs, the accuracies for T and N staging with MPR imaging were better than those with transverse imaging (each P < 0.05). In 34 eEGCs, however, only the accuracy of T2 or higher with MPR imaging was higher than that with transverse imaging (P < 0.05). CONCLUSIONS: Isotropic MDCT with MPR images including coronal or sagittal reconstructions can improve the accuracies of preoperative T and N staging of eAGC, while having little impact on the accuracy for eEGC.
Kim YN Choi D Kim SH Kim MJ Lee SJ Lee WJ Kim S Kim JJ
Department of Radiology and Center for Imaging Science, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Ilwon-dong, Kangnam-Ku, Seoul, 135-710, Republic of Korea.